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PCR Workshop in Kumasi, Ghana

CONVENTION OF BIOMEDICAL RESEARCH, GHANA (CoBReG)

CoBReG In Collaboration with the School Medicine & Dentistry, KNUST invite you to its 2nd PCR Workshop.

Date: Wed, 13th – Fri, 15th November 2019

Venue: Virus Research Laboratory, Dept of Microbiology, School of Medicine And Dentistry, KNUST

This is an introductory-level workshop suitable for those with little experience in molecular biology although some fore-knowledge is advisable. Prior experience with PCR is not a requirement. Participants will first learn key micro-pipetting techniques followed by the process of amplification in theory and proceed to techniques for PCR. Following this course, participants should be able to perform PCR reactions in their own laboratories, troubleshoot experiments, design primers and determine reaction conditions. We will cover critical requirements for amplification, thermostable DNA polymerases, primer design, gel electrophoresis, etc.

To Participate:

Send your Full Name, Department, and institution to akollor2001@yahoo.co.uk

participation Fee: GHC 100.00 (Pay by mobile money to 0240134805)

For Further Information, contact: by WhatsApp: 0505865492

Time

Wednesday

13th November 2019

Thursday

14th November 2019

Friday

15th November 2019

09:00-09:30am

Registration of participants

Recapitulation of day 1


Recapitulation of day 2

09:30-10:00am

Session 1: Welcome and introduction

Session 5: Principles underlying PCR/ Types of PCR/ Uses of PCR

Session 10: Preparation for electrophoresis

  • Preparation of buffer

  • Preparation of gel and tank

  • Casting of gel

10:00-10:15am

Break

Break

Break

10:15-10:45am

Session 2: Principles underlying DNA extraction & quantification (lectures and videos)

Stages in DNA extraction – Discussion of DNA extraction protocol(s)

(instructional videos)

Session 4: DNA extraction (practical

Session 6: Preparation for PCR

  • Preparation of master mix

  • Calculations


Session 7: Running the PCR

All facilitators

Session 11: Setting up electrophoresis

  • preparation of samples/

  • loading of samples and DNA ladder

running of gel

10:45am-11:00pm

Session 3:Liquid handling technique


ALL FACILITATORS



Session 8: Introduction to primer design and storage of PCR products

Session 12: Data Analysis

  • Capturing of image using Trans-illuminometer

  • Using software to analyse the band intensity


ALL FACILITATORS

11:00-2:00pm

Session 4:DNA extraction (practical) and Quantification


Session 9: Principles underlying agarose gel electrophoresis